Fig. 6. LPA delays the nuclear translocation of ERKs in a calcium-dependent manner. A. Serum starved Rat1 cells were treated with LPA (4 µg/ml) or EGF (50ng/ml) for the indicated times. Then, the lysates were subjected to Western blot analysis using anti-pERK or anti-gERK Ab as indicated. B. Following serum-starvation, Rat1 cells were treated with LPA (4 µg/ml; left) or EGF (50ng/ml) for the indicated times. Localization of ERKs and the nuclei were detected by staining with αgERK Ab or with DAPI, respectively. C. Serum-starved Rat-1 cells were treated with or without BAPTA-AM (BAPTA; 15min) prior to stimulation with LPA (4 µg/ml, 5 min). Localization of ERKs and nuclei was detected as in B.